POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY

The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono-or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in ligno...

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Hauptverfasser:	IANCU, Svetlana Laura, KABEL, Mirjam Anna, LANGE, Heiko, CRESTINI, Claudia, FROMMHAGEN, Matthias, KOETSIER, Martijn Johannes, BENJELLOUN-MLAYAH, Bouchra, VISSER, Jacob, GRUPPEN, Harm
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR IMPREGNATING OR COATING OF PAPER LAUNDERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PAPER NOT OTHERWISE PROVIDED FOR PAPER-MAKING PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D PRODUCTION OF CELLULOSE PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULP COMPOSITIONS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS TEXTILES TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G TREATMENT OF TEXTILES OR THE LIKE TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono-or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in lignocellulosic biomass. This newly discovered enzymatic activity renders these enzymes highly suitable for a plethora of applications in industry. Provided herein are methods of demethylation, processes to increase the reactivity of lignin or lignin-comprising biomass, processes of conversion lignin or lignin comprising biomass to value added products, processes for degrading and/or modifying (hemi-)cellulose in a hemicellulose-comprising substrate, and expression vectors, host cells and liquids, pastes or solid formulations and compositions for use in the demethylation method of the invention. La présente invention concerne des enzymes fongiques, en particulier des polyphénoloxydases (PPO), et se fonde sur une activité enzymatique nouvellement découverte d'une catégorie de PPO, c'est-à-dire la dé-méthylation de mono- ou di-méthoxyphénol R-substitué tel que présent dans la lignine, des composés dérivés de lignine et/ou en biomasse lignocellulosique. Cette activité enzymatique nouvellement découverte rend ces enzymes extrêmement appropriées pour une pléthore d'applications en industrie. L'invention concerne des procédés de déméthylation, des procédés pour augmenter la réactivité de lignine ou de biomasse comprenant de la lignine, des procédés de conversion de lignine ou de biomasse comprenant de la lignine en produits à valeur ajoutée, des procédés pour dégrader et/ou modifier la (hémi-)cellulose dans un substrat comprenant de l'hémicellulose, et des vecteurs d'expression, des cellules hôtes et des liquides, pâtes ou formulations solides et des compositions pour une utilisation dans le procédé de déméthylation de l'invention.