BIOTRANSFORMATION METHOD FOR TRANSFORMING PHENOLIC COMPOUNDS FROM SOY EXTRACT INTO EQUOL AND BIOACTIVE ISOFLAVONES BY FERMENTATION AND/OR ENZYMATIC APPLICATION, THUS OBTAINED COMPOSITION AND USE

The present invention relates to a biotransformation method for transforming phenolic compounds from soy milk into bioactive isoflavones and equol, with a high yield and production volume, for use in the biotechnological field, mainly in the food industry. The invention allows the glycosylated forms...

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Bibliographische Detailangaben
Hauptverfasser:	MACEDO, Gabriela Alves, DE QUEIRÓS, Lívia Dias, MACEDO, Juliana Alves
Format:	Patent
Sprache:	eng ; fre ; por
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HETEROCYCLIC COMPOUNDS HUMAN NECESSITIES HYGIENE MAKING THEREOF MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILK OR CHEESE SUBSTITUTES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a biotransformation method for transforming phenolic compounds from soy milk into bioactive isoflavones and equol, with a high yield and production volume, for use in the biotechnological field, mainly in the food industry. The invention allows the glycosylated forms of isoflavones (daidzin and genistin) to be biotransformed into the respective aglycone forms (daldzein and genistein) by fermentation of soy milk with initiator cultures and probiotic lactic bacteria, and/or by treatment of the extract with the enzyme tanase from Paecilomyces variotti. In one of the embodiments, the present invention relates to an in vitro method for producing bioactive equol in the absence of intestinal bacteria, using only enzymatic biotransformation. La présente invention concerne un procédé de biotransformation de composés phénoliques de l'extrait hydrosoluble de soja en isoflavones bioactives et en équol avec un haut rendement et une haute production, trouvant une application dans le domaine biotechnologique, notamment dans l'industrie alimentaire. L'invention a permis de biotransformer les formes glycosylées (daidzéine et genistéine) des isoflavones dans leurs formes aglycones respectives (daidzéine et génistéine) par fermentation de l'extrait hydrosoluble de soja avec des cultures initiatrices et des bactéries lactiques probiotiques et/ou par traitement dudit extrait avec l'enzyme tanase obtenue de Paecilomyces variotti. Dans l'un de ses modes de réalisation, la présente invention consiste en un procédé in vitro pour l'obtention d'équol bioactif, en l'absence de bactéries intestinales, seule la biotransformation enzymatique étant utilisée. A presente invenção refere-se a um processo de biotransformação de compostos fenólicos de extrato hidrossolúvel de soja em isoflavonas bioativas e equol com alto rendimento e alta produção para aplicação na área biolecnológica, principalmente para a Indústria alimentícia. A Invenção foi capaz de biotransformar as formas glicosiladas (daidzina e genistina) das Isoflavonas em suas respectivas formas agllconas (daldzeína e genlsteína) através de fermentação do extrato hidrossolúvel de soja com culturas Iniciadoras e bactérias lácticas probióticas e/ou tratamento do referido extrato com a enzima tanase obtida de Paecilomyces variotti. Em uma de suas concretizações, a presente invenção resulta em um processo in vitro para a obtenção de equol bioativo, sem a presença de bactérias intestinais, utilizando ape