MANIPULATION OF CELL NUCLEI IN A MICRO-FLUIDIC DEVICE

Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation regio...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	WHITE, Mark P, LOWE, Randall D., Jr
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS TRANSPORTING
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Beschreibung
Zusammenfassung:	Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process. Des aspects de la présente invention portent sur la manipulation d'un noyau cellulaire dans un dispositif microfluidique ainsi que sur des compositions, des systèmes et des kits permettant la mise en œuvre de tels procédés. Dans certains aspects, l'invention concerne des procédés permettant la disposition d'un ou plusieurs noyaux cellulaires choisis dans une zone d'isolement d'une enceinte de séquestration dans un dispositif microfluidique. Le ou les noyaux isolés peuvent ensuite être récupérés à partir de la zone d'isolement de l'enceinte de séquestration et utilisés dans un quelconque essai ou processus aval souhaité.