NEUROGENESIS OF DOPAMINERGIC NEURONS

The invention relates to a method of enhancing the differentiation of cells into neurons, wherein the cells are selected from the group multipotent stem cells, pluripotent stem cells and somatic cells, comprising seeding the cells onto a substrate patterned with nano- and/or micro-grating structures...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	SATHE, Sharvari Rajeev, TEO, Benjamin Kim Kiat, TAN, Kenneth Kian Boon, GOH, Lay Keow Eyleen, YIM, Evelyn King Fai, LIM, Keat Sandy, TANN, Jason Yong You
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICALDEVICES MICROSTRUCTURAL TECHNOLOGY MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method of enhancing the differentiation of cells into neurons, wherein the cells are selected from the group multipotent stem cells, pluripotent stem cells and somatic cells, comprising seeding the cells onto a substrate patterned with nano- and/or micro-grating structures, wherein the nano- and/or micro-grating structures are selected from gratings and hierarchical gratings, wherein said hierarchical gratings comprise said gratings with secondary structures at the base of and within the grooves of said gratings, and contacting the cells with medium; said substrates; a composite comprising at least one of said substrate, wells or chambers and either a plurality of said multipotent or pluripotent cells or a plurality of multipotent or pluripotent cell progeny, including cells differentiated therefrom; and a drug screening method involving the use of said composite. L'invention concerne un procédé d'amélioration de la différenciation de cellules en neurones, les cellules étant choisies dans le groupe des cellules souches multipotentes, des cellules souches pluripotentes et des cellules somatiques, consistant à ensemencer les cellules sur un substrat structuré par des structures de nanoréseau et/ou de microréseau, les structures de nanoréseau et/ou de microréseau étant choisies parmi les réseaux et les réseaux hiérarchiques, lesdits réseaux hiérarchiques comprenant lesdits réseaux avec des structures secondaire à la base de et à l'intérieur des rainures desdits réseaux, et à mettre en contact les cellules avec un milieu ; lesdits substrats ; un composite comprenant au moins l'un parmi ledit substrat, des puits ou des chambres et, soit une pluralité desdites cellules multipotentes ou pluripotentes, soit une descendance de la pluralité des cellules multipotentes ou pluripotentes, comprenant des cellules différenciées de celles-ci ; et un procédé de criblage de médicament impliquant l'utilisation dudit composite.