IMPROVED NGS WORKFLOW

The present invention relates to improved semi-automated methods that permit the extraction of nucleic acids from samples, preparation of PCR and post-PCR preparation steps of DNA- libraries for next-generation sequencings methods that can be conducted. The methods and additional aspects relating to...

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Bibliographische Detailangaben
Hauptverfasser:	ROUGH, SIOW, SAN, CHAO, LOU, PING, ZHANG, RUI, SEE, LEONG, TING, SMIRNOV, ARSENY, YONG, YEO, QIANG
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to improved semi-automated methods that permit the extraction of nucleic acids from samples, preparation of PCR and post-PCR preparation steps of DNA- libraries for next-generation sequencings methods that can be conducted. The methods and additional aspects relating to such methods are less laborious, safe costs, reagents and are less prone to contamination than comparable methods that are not automated. La présente invention concerne des procédés semi-automatiques améliorés qui permettent l'extraction d'acides nucléiques à partir d'échantillons, la préparation d'étapes de préparation de PCR et post-PCR de banques d'ADN pour des procédés de séquençage de nouvelle génération qui peuvent être pratiqués. Les procédés et autres aspects se rapportant aux dits procédés sont moins laborieux, moins coûteux, utilisent moins de réactifs et sont moins enclins à la contamination que des procédés comparables qui ne sont pas automatisé.