CODON-OPTIMIZED RECOMBINANT PHAGE AND METHODS OF USING SAME

Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimiz...

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Bibliographische Detailangaben
Hauptverfasser:	KRZYMANSKA-OLEJNIK, EDYTA, SHIVERS, ROBERT PATRICK, BROWNELL, DANIEL ROBERT, BOWERS, JAYSON L, CAPPILLINO, MICHAEL, KOERIS, MICHAEL SANDOR
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria. L'invention concerne une composition et des procédés de détection d'un ou de plusieurs microbes cibles. Les compositions de l'invention comprennent au moins un phage recombinant capable d'infecter un microbe cible, ledit phage comprenant au moins une séquence de protéine de capside, un site de liaison au ribosome et un marqueur à codons optimisés. Les compositions de l'invention peuvent, en outre, comprendre une solution aqueuse qui améliore la capacité de détection de l'expression du marqueur suite à une infection du microbe cible par le phage. Dans certains modes de réalisation, le microbe cible peut être Listeria.