PRODUCTION OF GLUCAN POLYMERS FROM ALTERNATE SUCROSE SOURCES

PRODUCTION OF GLUCAN POLYMERS FROM ALTERNATE SUCROSE SOURCES

Reaction solutions are disclosed herein comprising water, incompletely refined sucrose, and a glucosyltransferase enzyme that synthesizes insoluble poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages and a weight average degree of polymerization (DPw) of at least 100. The yield o...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HENNESSEY, Susan Marie, MATHER, Brian D, ADUGNA, Negash, FAKE, Dean M, ATKINSON, Wayne, OVENTAL, Jennifer S, PRITCHETT, Tyler D
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Reaction solutions are disclosed herein comprising water, incompletely refined sucrose, and a glucosyltransferase enzyme that synthesizes insoluble poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages and a weight average degree of polymerization (DPw) of at least 100. The yield of poly alpha-1,3-glucan by a reaction solution herein is at least 7% of the weight of sucrose that was converted in the reaction solution. Further disclosed are methods of producing poly alpha-1,3-glucan using incompletely refined sucrose, and poly alpha-1,3-glucan produced by these methods. L'invention concerne des solutions réactionnelles comprenant de l'eau, du saccharose raffiné de manière incomplète, et une enzyme glucosyltransférase qui synthétise un poly-alpha-1,3-glucane insoluble possédant au moins 50 % de liaisons alpha-1,3-glycosidiques et un degré de polymérisation moyen en poids (DPw) d'au moins 100. Le rendement en poly-alpha-1,3-glucane par une solution réactionnelle telle que décrite dans la description représente au moins 7 % du poids de saccharose qui a été converti dans la solution réactionnelle. L'invention concerne en outre des procédés de production de poly-alpha-1,3-glucane à l'aide de saccharose raffiné de manière incomplète, et de poly-alpha-1,3-glucane produit par ces procédés.