DETECTING INCREASE OR DECREASE IN THE AMOUNT OF A NUCLEIC ACID HAVING A SEQUENCE OF INTEREST

A method of detecting copy number variations in foetal or tumour DNA within a sample of circulating cell-free DNA includes carrying out amplification of DNA such that amplification of longer DNA molecules is discriminated against. This results in preferential amplification of foetal or tumour DNA, w...

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Bibliographische Detailangaben
Hauptverfasser:	REID, JAMES, CHITTY, LYN, HURD, DOUGLAS, LUEERSSEN, DIETRICH, SOUTHERN, EDWIN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of detecting copy number variations in foetal or tumour DNA within a sample of circulating cell-free DNA includes carrying out amplification of DNA such that amplification of longer DNA molecules is discriminated against. This results in preferential amplification of foetal or tumour DNA, which can then be analysed using, for example,array comparative genome hybridisation. L'invention concerne un procédé destiné à détecter des variations du nombre de copies d'ADN fœtal ou tumoral dans un échantillon d'ADN libre circulant, comprenant une amplification de l'ADN conduite de façon discriminante afin d'empêcher l'amplification de molécules d'ADN plus longues. Ce procédé conduit à l'amplification préférentielle de l'ADN fœtal ou tumoral, lequel peut ensuite être analysé, par exemple, par hybridation génomique comparative sur puce à ADN.