RECOMBINANT VECTOR FOR INCREASING BIOMASS AND LIPID PRODUCTIVITY OF MICROALGAE AND USE THEREOF

The present invention relates to: a recombinant vector comprising an inducible promotor induced under a condition in which ammonium is not present or a condition in which nitrate is present, and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein coding gene under the promotor; a method for p...

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Bibliographische Detailangaben
Hauptverfasser:	JEONG, BYEONG-RYOOL, SHIN, SUNG-EUN, JUN, SEUNG JIB, CHOI, GANG GUK, KWAK, MIN-SU, YANG, JI-WON, KANG, NAM KYU
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to: a recombinant vector comprising an inducible promotor induced under a condition in which ammonium is not present or a condition in which nitrate is present, and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein coding gene under the promotor; a method for producing transgenic microalgae having increased biomass and lipid productivity by transforming microalgae cells using the recombinant vector; transgenic microalgae produced by the method; a composition containing the recombinant vector as an active ingredient for increasing biomass and lipid productivity in microalgae; a method for producing lipid by cultivating the transgenic microalgae; and a method for manufacturing biodiesel by using the transgenic microalgae. La présente invention concerne : un vecteur recombiné comprenant un promoteur inductible, induit en l'absence d'ammonium ou en présence de nitrate, et un gène codant la protéine glycéraldéhyde-3-phosphate déshydrogénase (GAPDH) sous l'effet du promoteur ; un procédé de production de micro-algues transgéniques présentant une productivité de biomasse et de lipides augmentée par transformation de cellules de microalgues au moyen du vecteur recombiné ; des micro-algues transgéniques produites selon le procédé ; une composition contenant le vecteur recombiné en tant que principe actif pour augmenter la productivité de biomasse et de lipides dans des microalgues ; un procédé de production de lipides par culture des micro-algues transgéniques ; et un procédé de fabrication de biodiesel à l'aide des micro-algues transgéniques. 본 발명은 암모늄이 없는 조건 또는 질산염이 있는 조건에서 유도되는 유도성 프로모터 및 상기 프로모터 하위에 GAPDH(glyceraldehyde-3-phosphate dehydrogenase) 단백질 코딩 유전자를 포함하는 것을 특징으로 하는 재조합 벡터, 상기 재조합 벡터로 미세조류 세포를 형질전환하여 바이오매스와 지질 생산성이 증가된 형질전환 미세조류의 제조 방법, 상기 방법에 의해 제조된 형질전환 미세조류, 상기 재조합 벡터를 유효성분으로 포함하는, 미세조류의 바이오매스와 지질 생산성을 증가시키기 위한 조성물, 상기 형질전환 미세조류를 배양하여 지질을 생산하는 방법 및 상기 형질전환 미세조류를 이용한 바이오디젤의 제조 방법에 관한 것이다