RECOMBINANT LECTIN AND METHOD OF PREPARING THEREOF

The present invention provides a method for preparing a recombinant lectin expressed in a host cell such as E.coli or yeast, said method comprising the synthesis of a lectin gene based on amino acid sequence derived from the MALDI MS/MS of the Sclerotium rolfsii lectin (SEQ ID.1), a soil borne phyto...

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Bibliographische Detailangaben
Hauptverfasser:	S., RADHIKA, NAGRE, NAGARAJA NARAYAN, INAMDAR, SHASHIKALA RAMCHANDRA, CHACHADI, VISHWANATH BASAVARAJ, SWAMY, BALE MURUGI, GONCHIGAR, SATHISHA J, RAMADOSS, CANDALAI SESHADRI, VENKAT, HEMALATHA, MORYE, VINITA
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES
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Beschreibung
Zusammenfassung:	The present invention provides a method for preparing a recombinant lectin expressed in a host cell such as E.coli or yeast, said method comprising the synthesis of a lectin gene based on amino acid sequence derived from the MALDI MS/MS of the Sclerotium rolfsii lectin (SEQ ID.1), a soil borne phytopathogenic fungus, and its cloning in the host cell. La présente invention concerne un procédé de préparation d'une lectine recombinée exprimée dans une cellule hôte telle que E.coli ou la levure, ledit procédé comprenant la synthèse d'un gène de lectine sur la base d'une séquence d'acides aminés dérivée de la MALDI-MS/MS de la lectine de Sclerotium rolfsii (SEQ ID.1), un champignon phytopathogène du sol, et son clonage dans la cellule hôte.