HNL MUTANTS AT LOW PH

The present invention relates to newly identified mutants of (R)-selective hydroxynitrile lyase enzyme (R-HNL), in particular variants of Pa HNL (hydroxynitrile lyase isoenzyme 5 of Prunus amygdalus) which are stable at very low pH, i.e. at pH around 2.5 and lower. In particular, the present inventi...

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Bibliographische Detailangaben
Hauptverfasser:	ACHKAR, JIHANE, WIERTZ, ROEL WIM, CALLANT, DOMINIQUE MONIQUE CHARLES, WAECHTER, RALPH, PETSCHACHER, BARBARA, GLIEDER, ANTON, WEINHANDL, KATRIN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to newly identified mutants of (R)-selective hydroxynitrile lyase enzyme (R-HNL), in particular variants of Pa HNL (hydroxynitrile lyase isoenzyme 5 of Prunus amygdalus) which are stable at very low pH, i.e. at pH around 2.5 and lower. In particular, the present invention provides nucleic acids encoding said mutant enzymes, recombinant microorganisms and their use for industrial applications. La présente invention concerne des mutants nouvellement identifiés de l'enzyme lyase hydroxynitrile à sélectivité R (R-HNL), en particulier des variantes de l'enzyme Pa HNL (isoenzyme 5 lyase hydroxynitrile de prunus amygdalus) qui sont stables à un pH extrêmement bas, c'est-à-dire à un pH d'environ 2,5 ou moins. La présente invention concerne en particulier des acides nucléiques codant lesdites enzymes mutantes, des microorganismes recombinants et leur utilisation pour des applications industrielles.