UNIQUE BUFFERING SYSTEM FOR CELL CULTURE MEDIA AND GAMETE AND EMBRYO CULTURE MEDIA AND METHODS

The invention relates to compositions and methods for continuous stabilization of pH of cell culture medium notwithstanding change from one distinct environment to another environment. The methods include providing a buffering system that includes a sodium bicarbonate buffer and at least two zwitter...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	GILIAM, KIM, JOHN, MCMAHON, AIDAN, T, THOMPSON, JEREMY, G
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to compositions and methods for continuous stabilization of pH of cell culture medium notwithstanding change from one distinct environment to another environment. The methods include providing a buffering system that includes a sodium bicarbonate buffer and at least two zwitterionic buffers having concentrations significantly reduced as compared to conventionally used media buffers utilizing a single zwitterionic buffer. L'invention concerne des compositions et des procédés pour la stabilisation en continu du pH d'un milieu de culture cellulaire sans tenir compte d'une modification d'un environnement distinct à un autre environnement. Les procédés comprennent l'apport d'un système de tamponnage qui comprend un tampon bicarbonate de sodium et au moins deux tampons zwiterrioniques ayant des concentrations significativement réduites en comparaison à des tampons de milieu utilisés classiquement à l'aide d'un tampon zwitterionique unique.