CELL-FREE TRANSLATION SYSTEM

The present invention relates to a new cell-free translation system. In particular, the invention relates to a cell-free reaction system for translating in vitro a RNA into a protein, said reaction system comprising a ribosome-depleted red blood cell lysate and ribosomes isolated from eukaryotic cells, with the proviso that (1) when the ribosome-depleted red blood cell lysate is obtained from a nuclease untreated rabbit reticulocyte lysate, the eukaryotic cells from which ribosomes are isolated are not nuclease untreated rabbit reticulocytes, and (2) when the ribosome-depleted red blood cell lysate is obtained from a nuclease treated rabbit reticulocyte lysate, the eukaryotic cells from which ribosomes are isolated are not nuclease treated rabbit reticulocytes. The invention also pertains to a method for translating in vitro a ribonucleic acid template into an amino acid sequence of interest using the cell-free reaction system of the invention. The invention also relates to the use of (i) a ribosome-depleted red blood cell lysate, and (ii) ribosomes isolated from eukaryotic cells, with the proviso that (1) when the ribosome-depleted red blood cell lysate is obtained from a nuclease untreated rabbit reticulocyte lysate, the eukaryotic cells from which ribosomes are isolated are not nuclease untreated rabbit reticulocytes, and (2) when the ribosome-depleted red blood cell lysate is obtained from a nuclease treated rabbit reticulocyte lysate, the eukaryotic cells from which ribosomes are isolated are not nuclease treated rabbit reticulocytes, for producing a cell-free translation system. La présente invention concerne un nouveau système de traduction sans cellules. En particulier, l'invention concerne un système de réaction sans cellules permettant la traduction in vitro d'un ARN en une protéine, ledit système de réaction comprenant un lysat de globules rouges appauvris en ribosomes et des ribosomes isolés de cellules eucaryotes, sous réserve que (1) lorsque le lysat de globules rouges appauvris en ribosomes est obtenu à partir d'un lysat de réticulocytes de lapin non traités à la nucléase, les cellules eucaryotes à partir desquelles les ribosomes sont isolés ne sont pas des réticulocytes de lapin non traités à la nucléase et (2) lorsque le lysat de globules rouges appauvris en ribosomes est obtenu à partir d'un lysat de réticulocytes de lapin traités à la nucléase, les cellules eucaryotes à partir desquelles les ribosomes sont isolés ne sont pas des réticulo