A METHOD OF IMPROVING MICROARRAY PERFORMANCE BY STRAND ELIMINATION

A METHOD OF IMPROVING MICROARRAY PERFORMANCE BY STRAND ELIMINATION

The invention is a method of determining nucleotide sequence of a target nucleic acid using microarray analysis. Hybridization signals from probe sets corresponding to the sense and anti-sense strands are compared at each nucleotide position. If there is a substantial difference in performance betwe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
1. Verfasser: NAKAO, AKI
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention is a method of determining nucleotide sequence of a target nucleic acid using microarray analysis. Hybridization signals from probe sets corresponding to the sense and anti-sense strands are compared at each nucleotide position. If there is a substantial difference in performance between the two strands, probe sets from a poorly performing stand are eliminated from the sequence determination calculation for a particular nucleotide. L'invention est un procédé de détermination de la séquence nucléotidique d'un acide nucléique cible en utilisant une analyse sur puce à ADN. Les signaux d'hybridation de jeux de sondes correspondant aux brins sens et antisens sont comparés au niveau de chaque position de nucléotide. S'il existe une différence substantielle de performance entre les deux brins, les ensembles de sondes provenant d'un brin à faible performance sont éliminés du calcul de détermination de séquence pour un nucléotide particulier.