A METHOD OF IMPROVING THE ACTIVITY OF CELLULASE ENZYME MIXTURES IN THE SACCHARIFICATION OF (LIGNO)CELLULOSIC MATERIAL

The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: Chryso...

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Bibliographische Detailangaben
Hauptverfasser:	VISSER, JACOB, JUNDZIL, RICHARD, JOOSTEN, ROB, SINITSYN, ARKADY, KOETSIER, MARTIJN, WERY, JAN, EMALFARB, MARK
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: Chrysosporium, Thielavia, Talaromyces, Thermomyces, Thermoascus, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryplococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, Humicola, Trichoderma, and Talaromyces, plus anamorphs and teleomorphs thereof. Filamentous fungal organisms having improved activity profiles are obtained by modifying genes encoding enzymes involved in the production of cellobionolactone, cellobionic acid, gluconolactone, gluconic acid, and related products, by a variety of mutagenic methods, resulting in nucleotide substitutions, insertions, and deletions, increasing the level of saccharification in enzyme mixtures obtained from the modified organisms. La présente invention concerne des organismes fongiques filamenteux modifiés ayant des profils d'activité améliorés en ce qui concerne la conversion de glucides complexes en sucres simples à partir de matériaux cellulosiques, comprenant des organismes fongiques appartenant à un genre choisi dans le groupe constitué de : Chrysosporium, Thielavia, Talaromyces, Thermomyces, Thermoascus, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryplococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophtora, Mucor, Aspergillus, Fusarium, Humicola, Trichoderma, et Talaromyces, ainsi que des anamorphes et des téléomorphes de ceux-ci. Des organismes fongiques filamenteux ayant des profils d'activité améliorés sont obtenus par modification de gènes codant pour des enzymes impliquées dans la production de cellobionolactone, acide cellobionique, gluconolactone, acide gluconique, et des produits associés, par différents procédés de mutagenèse, conduisant à des substitutions, insertions, et délétions de nucléotide, augmentant le taux de saccharification dans des mélanges d'enzymes obtenus à partir des organismes modifiés.