METHOD FOR IMPROVED TRANSFORMATION USING AGROBACTERIUM

Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after expos...

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Bibliographische Detailangaben
1. Verfasser:	MILLER, PAUL DAVID
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Zusammenfassung:	Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after exposure to the Agrobacterium cells. Examples of plants useful with these methods include maize plants (e.g., immature maize embryos). L'invention concerne des procédés d'amélioration de la fréquence de transformation chez des plantes lorsque l'on utilise Agrobacterium comme transformant. Les procédés comprennent l'exposition de cellules végétales à des cellules d'Agrobacterium dans un milieu liquide contenant un agent tensio-actif. Certains procédés comprennent l'exposition des cellules végétales à de la lumière en continu après exposition aux cellules d'Agrobacterium. Des exemples de plantes utiles avec ces procédés comprennent les plants de maïs (par exemple les embryons de maïs immatures).