METHOD FOR QUANTITATIVE OPTICAL MEASUREMENTS AND LABORATORY APPARATUS

The invention is related to a method for the quantitative optical measurement of a char¬ acteristic property of at least one analyte in at least one laboratory sample, in particular for the fluorescence measurement of at least one biochemical or biological sample, the method using a laboratory apparatus, which has at least one light source and at least one detector device, the apparatus utilizing at least sensitivity parameter S, which controls the capability of the laboratory apparatus to detect a signal by means of the at lest one detector device, the method using source light for causing the at least one sample to emit a sample light, and the at least one detector device for detecting sample light and utilizing the at least one sensitivity parameter S to detect the corresponding at least one intensity I of the sample light, the method comprising the steps:- determining at least one reference point (S_ref; I ref); - using at least one first sensitivity parameter S_m1, which is not the same as S_ref, for measuring at least one first intensity I_m1 of sample light as-signed to a first analyte; -determining a quantity Q1, which is a measure for the slope of a line, which is determined by utilizing the at least one reference point (S_ref; l_ref) and the at least one measurement point (S_m1; I_m1 ); using the quantity Q1 for calculating a first analyte value C_m1, which is dependent on Q1 and which is characteristic for a property of the first analyte, in particular for a concentration of the first analyte in the at least one sample, in particular according to the formula Q1 = (I_m1 - l_ref) / (S_m1 - S_ref). The method, further, is related to a laboratory apparatus, which is configured to apply the method according to the invention. Cette invention concerne un procédé pour la mesure optique quantitative d'une propriété caractéristique d'au moins un analyte dans au moins un échantillon de laboratoire, en particulier, pour la mesure de la fluorescence d'au moins un échantillon biochimique ou biologique. Le procédé selon l'invention fait appel à un appareil de laboratoire, ledit appareil de laboratoire comprenant au moins une source de lumière et au moins un dispositif de type détecteur et utilisant au moins le paramètre de sensibilité S, qui régit la capacité de l'appareil de laboratoire à détecter un signal à l'aide dudit au moins dispositif de type détecteur. Le procédé selon l'invention utilise la lumière de la source pour que ledit au moins échantillon éme