METHOD AND APPARATUS FOR IMPLEMENTING A DYNAMICALLY SWITCHING DUPLEX MODE

The present invention provides an innovative versatile system, which allows delivery of one or several antigens or biologically active molecules into or onto targeted subset of cells. The invention is in particular directed to a combination of compounds and in particular to a composition, which comprises : (i) a fusion polypeptide comprising a streptavidin (SA) or avidin polypeptide and one or several effector molecule(s), wherein said fusion polypeptide retains the property of SA and avidin polypeptides to bind biotin; (ii) biotinylated targeting molecule(s), which are capable of targeting subset(s) of cells and/or cell surface molecule(s), and in particular dendritic cells (DC), subsets of DC and/or surface molecule(s) (including surface receptor(s)) of DC. The combination of the invention is suitable for use for targeting, in vivo, in vitro or ex vivo, of one or several effector molecule(s) to subset(s) of cells and/or cell surface molecule(s), and in particular for diagnosing or immunomonitoring a disease in a mammal or in prophylactic treatment and especially in vaccination and in therapy including in immunotherapy. The combination of the invention is also intended for use in vivo or ex vivo, for inducing a T cell immune response in bone marrow of naive donors before transplantation, or for activation and/or expansion of a T cell immune response in bone marrow of already immunized donors. The invention also relates to a method for the production of a fusion polypeptide of the invention and to a kit for a diagnostic test of a disease in a mammal, for immunomonitoring a disease in a mammal or for the prevention or treatment of a disease in a mammal. La présente invention se rapporte à un système polyvalent d'une grande inventivité, qui permet de délivrer un antigène ou une molécule biologiquement active, ou plus, à l'intérieur d'un sous-ensemble de cellules cible ou sur ledit sous-ensemble. De façon plus spécifique, l'invention se rapporte à une combinaison de composés et, plus particulièrement, à une composition qui comprend : (i) un polypeptide de fusion comprenant un polypeptide streptavidine (SA) ou avidine et une molécule effectrice ou plus, ledit polypeptide de fusion étant doté de la propriété des polypeptides SA et avidine à fixer la biotine ; (ii) une molécule de ciblage biotinylée ou plus, qui sont aptes à cibler un ou des sous-ensembles de cellules ou une ou des molécules de surface de cellule et, en particulier des cellules dendritiques (DC,