HIGH THROUGHPUT, OPTICAL METHOD AND SYSTEM FOR DETERMINING THE EFFECT OF A TEST SUBSTANCE ON LIVING CELLS

A rapid and efficient method and apparatus for detecting electrophysiologic, proarrhythmic, contractile, and other effects of substances such as compounds and drugs in native cellular cardiac preparations, the preparations representing an integrated cell-based pharmacologic response is disclosed. Mo...

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Hauptverfasser:	TRUMBULL, JONATHAN D, SU, ZHI, PAN, JEFFREY YEN, KANTOR, STANISLAW, DIAZ, GILBERT J, GREEN, JONATHON, OLSON, JEFFREY A, GINTANT, GARY A
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CALCULATING CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPUTING COUNTING ENZYMOLOGY HANDLING RECORD CARRIERS IMAGE DATA PROCESSING OR GENERATION, IN GENERAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PRESENTATION OF DATA RECOGNITION OF DATA RECORD CARRIERS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A rapid and efficient method and apparatus for detecting electrophysiologic, proarrhythmic, contractile, and other effects of substances such as compounds and drugs in native cellular cardiac preparations, the preparations representing an integrated cell-based pharmacologic response is disclosed. More specifically, a method to (1) rapidly and efficiently detect and verify the effects of chemicals, compounds and drugs on cardiac repolarization, contractility, and excitability using optically based techniques and customized simulation protocols, and (2) rapidly and efficiently screen and select compounds for electrophysiologic and proarrhythmic effects on cardiac myocytes is disclosed. La présente invention concerne un procédé rapide et efficace et un appareil pour détecter des effets électrophysiologiques, proarythmiques, contractiles, et autres de substances telles que des composés et des médicaments dans des préparations cardiaques cellulaires natives, les préparations représentant une réponse pharmacologique cellulaire. Plus spécifiquement, la présente invention concerne un procédé pour (1) détecter rapidement et efficacement et vérifier les effets de substances chimiques, composés et médicaments sur la repolarisation, la contractilité et l'excitabilité cardiaques en utilisant des techniques optiques et des protocoles de simulation personnalisés, et (2) cribler rapidement et efficacement et sélectionner des composés pour des effets électrophysiologiques et proarythmiques sur des myocytes cardiaques.