IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH FATTY ACID COMPOSITION IN PLANTS

A map was constructed from AFLP, RPLP and SSR analysis of the progeny derived from an interspecific cross involving a Colombian Elaeis oleifera (UP 1026) and a Nigerian E. guinneensis (T128). This interspecific cross was used to map genes associated with oil quality. A framework map was generated fo...

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Bibliographische Detailangaben
Hauptverfasser:	ABD. RAHMAN, RAHIMAH, HARMINDER SINGH, RAJINDER SINGH, OOI, CHENG LI, LESLIE, CHEAH, SUAN CHOO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PHYSICS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRAPPING VINEGAR WINE
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Zusammenfassung:	A map was constructed from AFLP, RPLP and SSR analysis of the progeny derived from an interspecific cross involving a Colombian Elaeis oleifera (UP 1026) and a Nigerian E. guinneensis (T128). This interspecific cross was used to map genes associated with oil quality. A framework map was generated for the male parent, T128, using Joinmap ver. 3.0. In the paternal guineensis map, 389 markers (305 AFLP, 64 RFLP and 20 SSR) were ordered in 18 linkage groups (157IcM). The E. guineensis map was also used in scanning for quantitative trait loci (QTLs) controlling oil quality (measured in terms of iodine value and fatty acid composition). At a 99 % significant genomic wide threshold level, QTLs associated with iodine value (IV), C14:0, C16:0, C16:l, C18:0 and C18:l were detected. The same genomic region appears to be influencing IV, Cl 8:1 and C 16:0 content. Considering the three traits are strongly correlated further supports the data observed. The QTLs also explain a significant proportion (about 35%) of the variation observed. Similarly significant QTLs for C16:l and C14:0 were detected around the same locus in separate genomic region. This probably points to another major locus influencing fatty acid composition. Markers associated with IV and FAC (C 14:0, C16:0, C16:l and Cl 8:1) were confirmed in a second mapping population or in palms of different genotype. The markers concerned are RFLP probes, namely CB75A (for IV, C16:0, C18:l) and RD56 (for C14:0 and C16:l) and microsatellite (SSR) marker P4T8 (Cl 8:0 and C16:0). Selon l'invention, une carte a été construite à partir d'une analyse par AFLP, RFLP et SSR de la descendance dérivant d'un croisement interspécifique impliquant un Elaeis oleifera colombien (UP 1026) et un E. guineensis nigériane (T128). Ce croisement interspécifique a été utilisé pour établir une carte des gènes associés à la qualité de l'huile. Une carte de référence a été produite pour le parent mâle, (T128), par utilisation de Joinmap ver. 3.0. Dans la carte de guineensis paternel, 389 marqueurs(305 AFLP, 64 RFLP et 20 SSR) ont été rangés en 18 groupes de liaison (157IcM). La carte de E. guineensis a elle aussi été utilisée pour explorer les loci des traits quantitatifs (QTL) qui commandent la qualité de l'huile (mesurée par l'indice d'iode et la composition en acides gras). A un niveau seuil sur l'ensemble du génome significatif de 99 %, on a détecté les QTL associés à l'indice d'iode (IV) et aux C14:0, C16:0, C16:1, C18:0 et C18:1. Cette