SYSTEMS AND METHODS FOR IDENTIFYING MICROPARTICLES

Disclosed are systems and methods for identifying microparticles or features arranged in high density arrays. Using the techniques of the present teachings allow for effective discrimination and characterization of microparticles such as sequencing beads or features having densities of about 39 x 10...

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Bibliographische Detailangaben
Hauptverfasser:	SHERIDAN, ANDREW, BEAUDOIN, ROBERT, E, YANG, CHENGYONG, GEHMAN, CURTIS, S
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CALCULATING CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING ENZYMOLOGY IMAGE DATA PROCESSING OR GENERATION, IN GENERAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are systems and methods for identifying microparticles or features arranged in high density arrays. Using the techniques of the present teachings allow for effective discrimination and characterization of microparticles such as sequencing beads or features having densities of about 39 x 106 particles/cm2 or more. In certain embodiments, such identification can be achieved via use of two or more images corresponding to respective subsets of the microparticles or features. In certain embodiments, microparticles in each such subset can be configured with a target that hybridizes with a labeled probe, thereby resulting in the corresponding image having lower density of objects to identify. The method of microparticle identification may encompass comparing of subset images with a common image, ranking of the microparticles to select those with the highest fluorescent identity. The invention further encompasses a system for biological analysis comprising a flow cell for receiving microparticles and a computer readable medium with instructions for identifying and ranking the microparticles. L'invention concerne des systèmes et des procédés d'identification de microparticules ou d'éléments agencés dans des réseaux haute densité. Les techniques selon l'invention permettent de différencier et de caractériser efficacement des microparticules telles que des perles ou éléments de séquençage présentant une densité d'environ 39 x 106 particules/cm2 ou supérieure. Dans certains modes de réalisation, l'identification peut être réalisée au moyen d'au moins deux images correspondant à des sous-ensembles respectifs de microparticules ou d'éléments. Dans certains modes de réalisation, des microparticules dans chaque sous-ensemble peuvent être configurées avec une cible qui s'hybride avec une sonde marquée, ce qui produit l'image correspondante présentant une densité inférieure d'objets à identifier.