PHOSPHOPEPTIDE ENRICHMENT OF COMPOSITIONS BY FRACTIONATION ON CERAMIC HYDROXYAPATITE

Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly h...

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Bibliographische Detailangaben
Hauptverfasser:	WALKER II, JOHN A, ACADEMIA, KATRINA, PAULUS, ARAN, LIU, NING, WEHR, TIM, FREEBY, STEVE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PEST REPELLANTS OR ATTRACTANTS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PLANT GROWTH REGULATORS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF SEPARATION THEIR RELEVANT APPARATUS TRANSPORTING TRAPPING
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Beschreibung
Zusammenfassung:	Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput. L'invention consiste à extraire des peptides phosphorylés de digestions de liquides biologiques et autres mélanges de peptides par fractionnement sur hydroxyapatite céramique. L'hydroxyapatite céramique est aisément utilisable dans une centrifugeuse, permettant des fractionnements rapides d'un grand nombre d'échantillons de faible volume et donc un rendement élevé.