STABLE BIOCATALYSTS OF PENICILLIN ACYLASE AS GEL AGGREGATES AND THE PROCESS OF MANUFACTURE THEREOF

The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross li...

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Hauptverfasser:	DATLA, ANUPAMA, PAVEL, KYSLIK, ASHAR, TRUPTI, KRISHNAKANT, STANISLAV, BECKA, VYASARAYANI, WILLIAMS, RAJASEKAR
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross linking agent like glutaraldehyde and further entrapped in a combination of natural polymer like gelatin and synthetic polymer like polyvinyl alcohol with effective gelation under mild conditions of temperature and pH, resulting in a stable biocatalyst. The enzymes immobilized by the above process include Penicillin acylase from rE.coli RE III (pKA18), Novel Penicillin acylase from Achromobacter sp (CCM4834) expressed in rE.coli BL21 (pK1P1) CCM 7394 and rE.coli RE III(pKX1P1). La présente invention concerne un biocatalyseur stable et viable ayant une activité et une stabilité foncitonnelle élevées et un procédé d'immobilisation de celui-ci. Le procédé d'immobilisation est basé sur le principe de piégeage de précipité d'enzyme partiellement purifiée qui est simultanément agrégé par un agent de réticulation tel que le glutaraldéhyde et additionnellement piégé dans une combinaison d'un polymère naturel tel que la gélatine et un polymère synthétique tel que l'alcool polyvinylique présentant une gélification efficace dans des conditions de température et de pH modérés, pour réaliser un biocatalyseur stable. Les enzymes immobilisées par le procédé ci-dessus comprennent la pénicilline acylase de rE.coli RE III (pKA18), une nouvelle pénicilline acylase de Achromobacter sp. (CCM4834) exprimée dans rE.coli BL21 (pKlPl) CCM 7394 et rE.coli RE III(pKXlPl).