METHOD FOR PURIFICATION OF VIRAL PROTEINS

The present invention relates to methods for obtaining viral proteins. In particular, the present invention relates to the purification of single viral membrane proteins from host cells, genetically engineered cells expressing viral membrane proteins and/or virus. Specifically, the present invention...

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Bibliographische Detailangaben
Hauptverfasser:	OPITZ, LARS, WOLFF, MICHAEL, REICHL, UDO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OF MACROMOLECULAR COMPOUNDS COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS POLYSACCHARIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to methods for obtaining viral proteins. In particular, the present invention relates to the purification of single viral membrane proteins from host cells, genetically engineered cells expressing viral membrane proteins and/or virus. Specifically, the present invention provides new methods wherein the host cells expressing viral membrane proteins recombinantly or host cells infected with virus and propagating the same are treated with surfactants for membrane solubilization of said host cells. La présente invention concerne des méthodes d'obtention de protéines virales. L'invention concerne en particulier la purification de protéines membranaires virales simples de cellules hôtes et de cellules obtenues par génie génétique exprimant des protéines membranaires virales et/ou des virus. La présente invention concerne plus spécifiquement de nouveaux procédés dans lesquels les cellules hôtes exprimant les protéines membranaires virales par recombinaison ou les cellules hôtes infectées par le virus et propageant celui-ci sont traitées à l'aide d'agents de surface pour la solubilisation membranaire desdites cellules hôtes.