METHOD TO STUDY PROLIFERATION OF ENDOTHELIAL PROGENITOR CELLS AND THE POTENTIAL INFLUENCE OF COMPOUNDS ON THEIR PROLIFERATION BEHAVIOUR

The present invention provides a simple and rapid method for determining colony forming units (CFU) of endothelial progenitor cells (EPC). Furthermore, a method for identifying compounds exerting an effect on those CFU of EPC is provided. The present invention also provides medium for culturing endo...

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Bibliographische Detailangaben
Hauptverfasser: BARTMANN, CHRISTINA, SCHALLMANN, KATHARINA, STRUNK, DIRK, ROHDE, EVA
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:The present invention provides a simple and rapid method for determining colony forming units (CFU) of endothelial progenitor cells (EPC). Furthermore, a method for identifying compounds exerting an effect on those CFU of EPC is provided. The present invention also provides medium for culturing endothelial cells which is comprised of EGM-2, hydrocortisone and VEGF as well as human platelet lysate (HPL) as supplement. La présente invention concerne un procédé simple et rapide pour déterminer les unités formant colonie (CFU) de cellules progénitrices endothéliales (EPC). De plus, l'invention concerne un procédé d'identification des composés exerçant un effet sur ces CFU d'EPC. La présente invention concerne également un milieu pour mettre en culture des cellules endothéliales, qui est composé d'EGM-2, d'hydrocortisone et de VEGF, ainsi que d'un lysat de plaquettes humaines (HPL) en tant que supplément.