NOVEL N-ACETYLGLUCOSAMINE-2-EPIMERASE AND METHOD FOR PRODUCING CMP-NEURAMINIC ACID USING THE SAME

The present invention relates to a novel N-acetylglucosamine-2- epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N- acetylneuraminic acid using...

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Hauptverfasser:	SOHNG, JAE KYUNG, GIL, TAE GEON, SEO, WON MIN, WOO, JIN SUK, KIM, BYUNG GEE, SHIM, SANG HEE, YANG, JI YOUNG, KIM, DEA HEE, JANG, KYOUNG SOON, HUR, IN KANG, KANG, SUN YOUP, JUNG, YOUNG SOO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a novel N-acetylglucosamine-2- epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N- acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates. L'invention concerne une nouvelle N-acétylglucosamine-2-épimérase et un procédé de préparation d'acide CMP-N acétylneuraminique. En particulier, l'invention concerne une N-acétylglucosamine-2-épimérase dérivée de Bacteroides fragilis NCTC 9343 et un procédé de préparation d'acide CMP-N acétylneuraminique faisant intervenir ladite N-acétylglucosamine-2-épimérase. Selon l'invention, l'acide CMP-N acétylneuraminique peut être produit en grande quantité, de manière économique, par une réaction comprenant une seule étape, à l'aide de monophosphate de cytidine et de N-acétyl-D-glucosamine qui constituent des substrats peu coûteux.