POLYMERASE VARIANTS FOR DNA SEQUENCING

Described herein is a mutant polymerase, specifically the Klenow exo- polymerase in which the proline at amino acid position 680 is replaced by a glycine (P680G), and its use in sequencing a single polynucleotide template using single molecule sequencing. L'invention concerne une polymérase mut...

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Bibliographische Detailangaben
1. Verfasser:	BUZBY, PHILIP, R
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described herein is a mutant polymerase, specifically the Klenow exo- polymerase in which the proline at amino acid position 680 is replaced by a glycine (P680G), and its use in sequencing a single polynucleotide template using single molecule sequencing. L'invention concerne une polymérase mutante, spécifiquement la Klenow exo-polymérase, dans laquelle la proline au niveau de la position d'acide aminé 680 est remplacée par une glycine (P680G), ainsi que son utilisation pour séquencer une matrice polynucléotide unique en utilisant un séquençage de molécule unique.