ARTIFICIAL CALIBRATION VIRUS TO CONTROL HIV VIRAL LOAD TESTS BY PCR IN REAL TIME

The present invention refers to the design of an artificial calibrating virus (ACV), as well as a methodology quality guarantee system, which has controlling characteristics in the performance of all the stages carried out during a detection and/or quantification molecular test. More specifically, t...

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Bibliographische Detailangaben
Hauptverfasser:	BRINDEIRO, PATRICIA ALVAREZ BAPTISTA, FERREIRA, ANTONIO GOMES PINTO DE, TANURI, AMILCAR, BRINDEIRO, RODRIGO DE MORAES BRINDEIRO, FERREIRA, JOSE ANTONIO PINTO DE SA
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention refers to the design of an artificial calibrating virus (ACV), as well as a methodology quality guarantee system, which has controlling characteristics in the performance of all the stages carried out during a detection and/or quantification molecular test. More specifically, the referred to ACV is used for the validation and calibration of quantitative determinations of circulating viruses in blood plasma samples by means of polymerase chain reaction (PCR) technology in real time (or 'real time PCR'). La présente invention concerne la conception d'un virus d'étalonnage artificiel (ACV) et un système de garantie de qualité de méthode, ce virus présentant des caractéristiques de contrôle en rapport avec la performance de toutes les étapes réalisées pendant un test moléculaire de détection et/ou de quantification. Plus particulièrement, ledit ACV est utilisé pour la validation et l'étalonnage de déterminations quantitatives de virus en circulation dans des échantillons de plasma sanguin au moyen d'une technique de réaction en chaîne de la polymérase (PCR) en temps réel (ou 'PCR en temps réel').