UTILIZATION OF COMPATIBLE SOLUTES TO IMPROVE THE PERFORMANCE OF THE TECHNIQUES USING IMMOBILIZED BIOLOGIC MATERIALS

This invention is based upon the application of compatible solutes to immobilized biological materials or to immobilize in order to improve the technical performance using the same, namely nucleic acid and proteins or biosensors micronets. The above mentioned compatible solutes are selected from a g...

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Hauptverfasser:	LOOSE, ELISABETH, LAMOSA ANTONIO, PEDRO, MACHADO, CATIA, MAYCOCK, CHRISTOPHER DAVID, PEREIRA DE OLIVEIRA DA SILVA SANTOS, JORGE MIGUEL, MENDES BORDALO VENTURA CENTENO LIMA, MARIA RITA, VARELA RAPOSO, ANA CATARINA, SEQUEIRA LOPES, ISABEL, SETFORD, STEVEN JOHN, VOLINIA, STEFANO, MASCELLANI, NICOLETTA, SANTOS, HELENA, DE LEAO E FLORES, ALCINO ORFEU
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention is based upon the application of compatible solutes to immobilized biological materials or to immobilize in order to improve the technical performance using the same, namely nucleic acid and proteins or biosensors micronets. The above mentioned compatible solutes are selected from a group that includes Mannosylglycerate (MG) and its 3 derivatives manno- sylglyceramide (MGA), -mannosylglycerol (MGOH), and mannosyl-lactate (MGLac), 1,1-di-glycerol phosphate (DGP), ectoin, hydroxyectoin, and or derivatives or combinations thereof. The improved performance of the above mentioned techniques might be due to: a better state of preservation of the material to be immobilized, increasing the life expectancy of the material, and/or decreasing the storage and/or transport requirements, a more efficient and uniform deposition of the material on the support, more efficiency and/or specificity of hybridation of the marked material, all this originating an increase of the signal-to-noise ratio. L'invention concerne l'application de solutés compatibles sur des matières biologiques immobilisées ou pour entraîner une immobilisation en vue d'améliorer l'efficacité technique au moyen de cette application, notamment de microréseaux et de biodétecteurs basés sur des acides nucléiques et des protéines. Les solutés susmentionnés sont sélectionnés parmi un groupe comprenant le mannosylglycérate (MG) et ses trois dérivés mannosylglycéramide (MGA), -mannosylglycérol (MGOH), et mannosyl-lactate (MGLac), 1,1-di-glycérol phosphate (DGP), l'ectoïne, l'hydroxyectoïne et/ou des dérivés ou des combinaisons de ceux-ci. L'efficacité améliorée des techniques susmentionnées peut provenir d'un meilleur état de conservation de la matière à immobiliser, de l'augmentation de la durée de vie de la matière et/ou la diminution des exigences en matière de stockage et/ou de transport, d'un dépôt plus efficace et uniforme de la matière sur le support, d'une plus grande efficacité et/ou spécificité de l'hybridation de la matière marquée. Tout ceci provoque donc une augmentation du rapport signal/bruit.