METHOD FOR EXTRACTION AND IDENTIFICATION OF NUCLEIC ACIDS

The invention provides a method for extracting nucleic acids from a sample. The sample contains cells, viruses, or both cells and viruses. The method include adding a lysing solution containing a detergent to the sample to lyse the cells or viruses to form a lysate, adding alcohol to the lysate to a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	ANDRUS, LINDA, LEE, DON-HUN, PFAHLER, WOLFRAM, H.,E, PRINCE, ALFRED, M
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a method for extracting nucleic acids from a sample. The sample contains cells, viruses, or both cells and viruses. The method include adding a lysing solution containing a detergent to the sample to lyse the cells or viruses to form a lysate, adding alcohol to the lysate to aggregate or precipitate the nucleic acids, and purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber filter. L'invention concerne une méthode permettant d'extraire des acides nucléiques d'un échantillon. Ledit échantillon contient des cellules, des virus ou les deux. La méthode consiste à ajouter une solution de lyse contenant un détergent à l'échantillon afin de lyser les cellules ou les virus et de former un lysat, à ajouter un alcool au lysat afin d'agréger ou de précipiter les acides nucléiques et à purifier les acides nucléiques provenant du mélange lysat-alcool par filtrage dudit mélange à travers un filtre de fibre de verre.