ENHANCED DETECTION OF RNA USING A PANEL OF TRUNCATED GENE-SPECIFIC PRIMERS FOR REVERSE TRANSCRIPTION

The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are...

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Bibliographische Detailangaben
Hauptverfasser:	COLES, DAVID, MITAS, MICHAEL, CHIAFARI, FRANCIS, ALLEN, ANTONETTE C
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided. L'invention concerne des amorces spécifiques à un gène tronqué dans des ensembles pouvant être utilisés durant l'étape de transcription inverse de RT-PCR afin d'améliorer la détection de signal des marqueurs géniques du cancer dans un échantillon de tissu. L'invention concerne également des amorces inverses et directes pour RT-PCR. L'invention concerne enfin des procédés d'utilisation de ces amorces.