BIOTIN-FACILITATED TRANSPORT INTO GRAM NEGATIVE BACTERIA

The invention provides a method for biotin-facilitated introduction of a compound into a bacterial cell, preferably a Gram negative bacterial cell. The compound to be introduced into the cell is biotinylated, and the biotinylated compound is contacted with the cell to effect delivery of the compound to the cell. Advantageously, the biotinylated compound can pass through both the inner and outer cell membranes and is delivered to the cytosol of the cell. A biotinylated compound can be delivered to any Gram negative bacterial cell capable of transporting biotin from the extracellular environment to the intracellular environment. L'invention concerne la biotinylation de composés, tels que des peptides et des peptidomimétiques, qui facilite le transport illicite desdits composés dans des bactéries gram négatif.