CONSTRUCTION OF A GST-CBD FUSION PROTEIN HAVING SEVERAL ENZYMATIC ACTIVITIES

The invention relates to the construction, purification and characterisation of a GST-CBD fusion protein. The inventive protein has very high and very diverse degradative enzymatic activities: cellulase, beta-glucosidase, xylanase, chitinase, laccase, while retaining the original Glutathione S trans...

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Bibliographische Detailangaben
Hauptverfasser:	AMOURI, BAHIA, GARGOURI, ALI
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS DYEING LEATHER, FURS, OR SOLID MACROMOLECULAR SUBSTANCES INANY FORM DYEING OR PRINTING TEXTILES ENZYMES ENZYMOLOGY FATTY ACIDS THEREFROM FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR LAUNDERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS TEXTILES TREATMENT OF TEXTILES OR THE LIKE TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the construction, purification and characterisation of a GST-CBD fusion protein. The inventive protein has very high and very diverse degradative enzymatic activities: cellulase, beta-glucosidase, xylanase, chitinase, laccase, while retaining the original Glutathione S transferase activity thereof. All of the aforementioned potentialities are highly desirable on an industrial scale. L'invention concerne la construction, la purification et la caractérisation d'une protéine de fusion « GST-CBD ». Cette protéine possède de très fortes activités enzymatiques degradatives très diverses : cellulase, beta-glucosidase, xylanase, chitinase, laccase, tout en gardant son activité originelle de Glutathion S transférase. Toutes ces potentialités sont très recherchées à l'échelle industrielle.