METHOD OF PURIFYING MODIFIED ACARIAN MAIN ALLERGEN

METHOD OF PURIFYING MODIFIED ACARIAN MAIN ALLERGEN

A method of purifying a modified acarian main allergen obtained by a technology of genetic manipulation; and a modified acarian main allergen having been purified by the purification method. In particular, a method of purifying a modified acarian main allergen obtained by a technology of genetic man...

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Hauptverfasser: MURAKAMI, TOSHIO, MAEDA, TOSHIHIRO, KAWATSU, KENJIRO, MIYATSU, YOSHINOBU, KOYANAGI, SATOSHI, MIZOKAMI, HIROSHI
Format: Patent
Sprache:eng ; fre ; jpn
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method of purifying a modified acarian main allergen obtained by a technology of genetic manipulation; and a modified acarian main allergen having been purified by the purification method. In particular, a method of purifying a modified acarian main allergen obtained by a technology of genetic manipulation, which method is characterized by comprising the steps of (1) with the use of an MF membrane, washing and recovering an inclusion body containing a modified acarian main allergen obtained by a technology of genetic manipulation, (2) dissolving the inclusion body and performing refolding, (3) with the use of an ultrafiltration membrane, concentrating the resultant modified acarian main allergen containing solution and removing low-molecular components, (4) with the use of an anion exchanger, recovering the modified acarian main allergen as a non-adsorbed fraction, (5) with the use of hydrophobic gel, recovering the modified acarian main allergen as an adsorbed fraction and (6) with the use of an anion exchanger, recovering the modified acarian main allergen as an adsorbed fraction; and a modified acarian main allergen of high purity obtained by the purification method. L'invention concerne un procédé de purification d'allergène principal d'acarien modifié par manipulation génétique, et elle concerne aussi cet allergène ainsi modifié, et en particulier un procédé de purification dudit allergène obtenu par manipulation génétique, selon les étapes suivantes : (1) avec une membrane MF, lavage et récupération d'un corps d'inclusion renfermant ledit allergène obtenu par manipulation génétique, (2) dissolution de ce corps et repliage, (3) avec une membrane d'ultrafiltration, concentration de la solution résultante renfermant l'allergène en question et extraction des composantes de faible poids moléculaire, (4) avec un échangeur anionique, extraction de l'allergène considéré en tant que fraction non adsorbée, (5) avec un gel hydrophobe, extraction du même allergène en tant que fraction adsorbée, et (6) avec un échangeur anionique, extraction de cet allergène en tant que fraction adsorbée. L'invention concerne également cet allergène à haute pureté obtenu par le procédé de purification décrit.