GENE-CODING POLYNUCLEOTIDES OF THE CHROMOSOME OF THE BACTERIUM CHROMOBACTERIUM VIOLACEUM, EXPRESSION AND ACTIVITY OF THESE POLYNUCLEOTIDES AND THEIR APPLICATIONS

Chromobacterium violaceum has developed the capacity to adapt to abrupt environmental changes, including in relation to the availability of nutrients, temperature changes and the presence of toxic elements, among other factors. Different genes of this organism have been identified and all the transaction mechanisms, such as the RNA polymers and the sigma factors, have been annotated, such as . (rpoD), . (rpoN), . (rpoH), . (rpoS), . (fliA), . (rpoE) and anti-. factor (flgM), related to . (yhbH) and Ecf-. . In addition to this, a variety of transcriptional activators and repressors that interact with the sigma factors and are involved in the response of the bacterium to environmental stress are also present, with various genes related to the families LysR, AraC, TetR, GntR, Mar, Mer, AsnC, DeoR, AsrR, Lacl and Crp, among others. Even more significant has been the detection of a high number of regulatory proteins associated with the adaptation to different environments, representing almost 9% of the genes of Chromobacterium violaceum. The versatility of Chromobacterium violaceum has also been brought into evidence in the more than 300 genes identified and related to its energy metabolism, which allow it to obtain energy under aerobic, microaerobic and anaerobic conditions. Genes related to the absorption of certain nutrients via mechanisms that are known to confer advantages on the bacterium in its competition against other microorganisms have also been detected. Further more, various genes that are capable of favoring survival under conditions of nutrient limitation have been detected, such as the genes involved in the utilization of peptides under carbon stress, the proteins SspA and SspB that are induced because of glycose, nitrogen, phosphate and amino acid deficiencies, the universal stress proteins (USP) that normally act under the stresses of high temperatures, salinity and exposure to ultraviolet light, among others. Certain genome functions have been established. Chromobacterium violaceum a développé la capacité de s'adapter à des brusques variations environnementales, notamment par rapport à la disponibilité des nutriments, aux variations de température et à la présence d'éléments toxiques parmi d'autres facteurs. Différents gènes de cet organisme ont été identifiés et tous les mécanismes de transcription, tels que les polymères d'ARN et les facteurs sigma, ont été annotés, tels que (rpoD), (rpoN), (rpoH), (rpoS), (fliA), (rpoE) et anti- facteur (f