METHODS AND COMPOSITIONS FOR DETECTION AND QUANTITATION OF NUCLEIC ACID ANALYTES

The present invention provides novel solution phase hybridization-based methods for detecting and quantitating nucleic acid analytes. Methods comprising use of novel capture polymers and/or signaling systems are provided. Use of these novel capture polymers and/or signaling systems provides signific...

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Bibliographische Detailangaben
Hauptverfasser:	BILLECI, TODD, WONG, WAI LEE TAN, TSAI, SIAO PING, STEPHAN, JEAN-PHILIPPE, F
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides novel solution phase hybridization-based methods for detecting and quantitating nucleic acid analytes. Methods comprising use of novel capture polymers and/or signaling systems are provided. Use of these novel capture polymers and/or signaling systems provides significant improvements in signal to noise ratio, specificity, sensitivity and ease of development and use as compared to existing solution phase nucleic acid detection and quantitation methods. The invention further provides compositions, kits and articles of manufacture for practicing methods of the present invention. L'invention porte sur de nouveaux procédés à base d'hybridation de détection et quantification d'analytes d'acides nucléiques en phase soluble comprenant l'utilisation de nouveaux polymères de capture et/ou systèmes de signalisation. L'utilisation de ces nouveaux polymères apporte une amélioration significative du rapport signal/bruit, de la spécificité, de la sensibilité, et de la facilité de développement et d'utilisation, par rapport aux procédés actuels de détection et quantification d'acides nucléiques en phase soluble. L'invention porte en outre sur des compositions, des trousses et des articles manufacturés de mise en pratique des procédés de l'invention.