TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE
The invention features a transgenic non-human mammal expressing or capable of expressing a GSK-3 transgene comprising a GSK-3 coding sequence, wherein expression of said coding sequence in the mammal is driven by an operably linked regulatory sequence that directs inducible expression in muscle. Fol...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | GAROFALO, ROBERT, SEBASTIAN SCHACHTER, JOEL, BARRY ORENA, STEPHEN, JOSEPH |
| description | The invention features a transgenic non-human mammal expressing or capable of expressing a GSK-3 transgene comprising a GSK-3 coding sequence, wherein expression of said coding sequence in the mammal is driven by an operably linked regulatory sequence that directs inducible expression in muscle. Following the induction of transgene expression, the transgenic non-human mammal exhibits hyperinsulinemia, increased weight gain on a high fat diet, and/or decreased muscle glycogen content on a high fat, as compared to a control animal not expressing the GSK-3 transgene. The invention also features methods of identifying in vivo modulators of GSK-3 activity.
L'invention concerne un mammifère non humain transgénique exprimant ou capable d'exprimer un trangène de la GSK-3 comprenant une séquence de codage de la GSK-3, l'expression de cette séquence de codage chez le mammifère étant commandée par une séquence régulatrice fonctionnellement liée qui dirige l'expression inductible dans le muscle. Suite à l'induction de l'expression du transgène, le mammifère non humain transgénique présente une hyperinsulinémie, un gain pondéral accru pour un régime cétogène et/ou une teneur réduite de glycogène dans le muscle pour un régime cétogène comparé à un animal témoin n'exprimant pas le transgène de la GSK-3. L'invention concerne également des procédés d'identification de modulateurs in vivo de l'activité GSK-3. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO03053138A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO03053138A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO03053138A13</originalsourceid><addsrcrecordid>eNrjZLANCXL0C3Z39fN0VnCNCAhyDQ729PdT8HdTcPeJdPYHSigER_qFeDgGuyp4e_qBKGMFTz8F39BgZx9XHgbWtMSc4lReKM3NoOjmGuLsoZtakB-fWlyQmJyal1oSH-5vYGxgamxobOFoaEyMGgA59Snm</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE</title><source>esp@cenet</source><creator>GAROFALO, ROBERT, SEBASTIAN ; SCHACHTER, JOEL, BARRY ; ORENA, STEPHEN, JOSEPH</creator><creatorcontrib>GAROFALO, ROBERT, SEBASTIAN ; SCHACHTER, JOEL, BARRY ; ORENA, STEPHEN, JOSEPH</creatorcontrib><description>The invention features a transgenic non-human mammal expressing or capable of expressing a GSK-3 transgene comprising a GSK-3 coding sequence, wherein expression of said coding sequence in the mammal is driven by an operably linked regulatory sequence that directs inducible expression in muscle. Following the induction of transgene expression, the transgenic non-human mammal exhibits hyperinsulinemia, increased weight gain on a high fat diet, and/or decreased muscle glycogen content on a high fat, as compared to a control animal not expressing the GSK-3 transgene. The invention also features methods of identifying in vivo modulators of GSK-3 activity.
L'invention concerne un mammifère non humain transgénique exprimant ou capable d'exprimer un trangène de la GSK-3 comprenant une séquence de codage de la GSK-3, l'expression de cette séquence de codage chez le mammifère étant commandée par une séquence régulatrice fonctionnellement liée qui dirige l'expression inductible dans le muscle. Suite à l'induction de l'expression du transgène, le mammifère non humain transgénique présente une hyperinsulinémie, un gain pondéral accru pour un régime cétogène et/ou une teneur réduite de glycogène dans le muscle pour un régime cétogène comparé à un animal témoin n'exprimant pas le transgène de la GSK-3. L'invention concerne également des procédés d'identification de modulateurs in vivo de l'activité GSK-3.</description><edition>7</edition><language>eng ; fre</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CARE OF BIRDS, FISHES, INSECTS ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW BREEDS OF ANIMALS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2003</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20030703&DB=EPODOC&CC=WO&NR=03053138A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20030703&DB=EPODOC&CC=WO&NR=03053138A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GAROFALO, ROBERT, SEBASTIAN</creatorcontrib><creatorcontrib>SCHACHTER, JOEL, BARRY</creatorcontrib><creatorcontrib>ORENA, STEPHEN, JOSEPH</creatorcontrib><title>TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE</title><description>The invention features a transgenic non-human mammal expressing or capable of expressing a GSK-3 transgene comprising a GSK-3 coding sequence, wherein expression of said coding sequence in the mammal is driven by an operably linked regulatory sequence that directs inducible expression in muscle. Following the induction of transgene expression, the transgenic non-human mammal exhibits hyperinsulinemia, increased weight gain on a high fat diet, and/or decreased muscle glycogen content on a high fat, as compared to a control animal not expressing the GSK-3 transgene. The invention also features methods of identifying in vivo modulators of GSK-3 activity.
L'invention concerne un mammifère non humain transgénique exprimant ou capable d'exprimer un trangène de la GSK-3 comprenant une séquence de codage de la GSK-3, l'expression de cette séquence de codage chez le mammifère étant commandée par une séquence régulatrice fonctionnellement liée qui dirige l'expression inductible dans le muscle. Suite à l'induction de l'expression du transgène, le mammifère non humain transgénique présente une hyperinsulinémie, un gain pondéral accru pour un régime cétogène et/ou une teneur réduite de glycogène dans le muscle pour un régime cétogène comparé à un animal témoin n'exprimant pas le transgène de la GSK-3. L'invention concerne également des procédés d'identification de modulateurs in vivo de l'activité GSK-3.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CARE OF BIRDS, FISHES, INSECTS</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW BREEDS OF ANIMALS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2003</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLANCXL0C3Z39fN0VnCNCAhyDQ729PdT8HdTcPeJdPYHSigER_qFeDgGuyp4e_qBKGMFTz8F39BgZx9XHgbWtMSc4lReKM3NoOjmGuLsoZtakB-fWlyQmJyal1oSH-5vYGxgamxobOFoaEyMGgA59Snm</recordid><startdate>20030703</startdate><enddate>20030703</enddate><creator>GAROFALO, ROBERT, SEBASTIAN</creator><creator>SCHACHTER, JOEL, BARRY</creator><creator>ORENA, STEPHEN, JOSEPH</creator><scope>EVB</scope></search><sort><creationdate>20030703</creationdate><title>TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE</title><author>GAROFALO, ROBERT, SEBASTIAN ; SCHACHTER, JOEL, BARRY ; ORENA, STEPHEN, JOSEPH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO03053138A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2003</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CARE OF BIRDS, FISHES, INSECTS</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW BREEDS OF ANIMALS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GAROFALO, ROBERT, SEBASTIAN</creatorcontrib><creatorcontrib>SCHACHTER, JOEL, BARRY</creatorcontrib><creatorcontrib>ORENA, STEPHEN, JOSEPH</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GAROFALO, ROBERT, SEBASTIAN</au><au>SCHACHTER, JOEL, BARRY</au><au>ORENA, STEPHEN, JOSEPH</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE</title><date>2003-07-03</date><risdate>2003</risdate><abstract>The invention features a transgenic non-human mammal expressing or capable of expressing a GSK-3 transgene comprising a GSK-3 coding sequence, wherein expression of said coding sequence in the mammal is driven by an operably linked regulatory sequence that directs inducible expression in muscle. Following the induction of transgene expression, the transgenic non-human mammal exhibits hyperinsulinemia, increased weight gain on a high fat diet, and/or decreased muscle glycogen content on a high fat, as compared to a control animal not expressing the GSK-3 transgene. The invention also features methods of identifying in vivo modulators of GSK-3 activity.
L'invention concerne un mammifère non humain transgénique exprimant ou capable d'exprimer un trangène de la GSK-3 comprenant une séquence de codage de la GSK-3, l'expression de cette séquence de codage chez le mammifère étant commandée par une séquence régulatrice fonctionnellement liée qui dirige l'expression inductible dans le muscle. Suite à l'induction de l'expression du transgène, le mammifère non humain transgénique présente une hyperinsulinémie, un gain pondéral accru pour un régime cétogène et/ou une teneur réduite de glycogène dans le muscle pour un régime cétogène comparé à un animal témoin n'exprimant pas le transgène de la GSK-3. L'invention concerne également des procédés d'identification de modulateurs in vivo de l'activité GSK-3.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO03053138A1 |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE |
| title | TRANSGENIC EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN MUSCLE |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T13%3A54%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=GAROFALO,%20ROBERT,%20SEBASTIAN&rft.date=2003-07-03&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO03053138A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |