REAL-TIME MONITORING OF PCR AMPLIFICATION USING NANOPARTICLE PROBES

The present invention relates to the use of nanoparticle detection probes to monitor amplification reactions, especially polymerase chain reactions ("PCR"). More specifically, the present invention involves the use of nanoparticles oligonucleotide conjugates treated with a protective agent...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	FRITZ, BRETT, M, STORHOFF, JAMES, J, HERRMANN, MARK
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention relates to the use of nanoparticle detection probes to monitor amplification reactions, especially polymerase chain reactions ("PCR"). More specifically, the present invention involves the use of nanoparticles oligonucleotide conjugates treated with a protective agent such as bovine serum albumin in an homogeneous assay format in order to quantitatively and qualitatively detect a target polynucleotide. La présente invention concerne l'utilisation de sondes de détection nanoparticulaires pour suivre les réactions d'amplification, spécialement les réactions d'amplification en chaîne par polymérase (PCR). L'invention concerne plus particulièrement l'utilisation de conjugués d'oligonucléotides particulaires traités avec un agent protecteur tel que l'albumine sérique bovine dans un format d'essai homogène de façon à détecter quantitativement et qualitativement un polynucléotide cible.