SYSTEM FOR PRODUCING CLONAL OR COMPLEX POPULATIONS OF RECOMBINANT ADENOVIRUSES, AND THE APPLICATION OF THE SAME

The invention relates to a novel system for producing recombinant adenoviruses (rAd). The areas of application of said system are medicine, veterinary medicine, biotechnology, genetic engineering, and functional genomic analysis. The inventive system for producing rAds preferably consists of a donor virus, the packaging signal of which is (i) partially deleted and (ii) is surrounded by parallel recognition sites for a site-specific recombinase; a packaging cell line which expresses the site-specific recombinase; and donor plasmids containing (i) at least one recognition site for the site-specific recombinase, (ii) the full viral packaging signal, (iii) optionally two recognition sites for a rarely cutting restriction endonuclease, and (iv) insertion sites for foreign DNA or inserted foreign DNA. The invention relates to a novel system for producing recombinant adenoviruses (rAd). The areas of application of said system are medicine, veterinary medicine, biotechnology, genetic engineering, and functional genomic analysis. The inventive system for producing rAds preferably consists of a donor virus, the packaging signal of which is (i) partially deleted and (ii) is surrounded by parallel recognition sites for a site-specific recombinase; a packaging cell line which expresses the site-specific recombinase; and donor plasmids containing (i) at least one recognition site for the site-specific recombinase, (ii) the full viral packaging signal, (iii) optionally two recognition sites for a rarely cutting restriction endonuclease, and (iv) insertion sites for foreign DNA or inserted foreign DNA. Die Erfindung betrifft ein neuartiges System zur Herstellung rekombinanter Adenoviren (rAd); Einsatzgebiete sind die Medizin, Veterinärmedizin, Biotechnologie, Gentechnik und die funktionelle Genomanalyse. Das erfindungsgemässe System zur rAd-Herstellung besteht bevorzugt aus - einem Donorvirus, dessen Verpackungssignal (i) partiell deletiert ist und (ii) von parallel orientierten Erkennungsstellen für eine ortsspezifische Rekombinase umrahmt wird, - einer Verpackungszelllinie, die die ortsspezifische Rekombinase exprimiert und Donorplasmiden, die (i) eine oder zwei Erkennungsstellen für die ortspezifische Rekombinase, (ii) das vollständige virale Verpackungssignal, (iii) gegebenenfalls zwei Erkennungsstellen für eine selten schneidende Restriktionsendonuklease und (iv) insertionsstellen für Fremd-DNA bzw. insertierte Fremd-DNA enthalten. La présente invention concerne un n