PROCESS FOR OBTAINING GROWTH FACTOR (TGF-BETA AND IGF-1), LACTOPEROXIDASE AND IMMUNOGLOBULINS PREPARATIONS FROM MILK PRODUCTS HAVING LOW MUTUAL CROSS-CONTAMINATION

The present invention relates to a process for extracting beneficial compounds, in particular growth factors, such as TGF beta and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydr...

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Bibliographische Detailangaben
Hauptverfasser:	GALAMA, CATHARINA, MARINA, KIVITS, MARINUS, GERARDUS, CORNELIS, HENDRIKS, ANDOR, WILHELM, JOSEPH
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROTEIN COMPOSITIONS FOR FOODSTUFFS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE WORKING-UP PROTEINS FOR FOODSTUFFS
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Beschreibung
Zusammenfassung:	The present invention relates to a process for extracting beneficial compounds, in particular growth factors, such as TGF beta and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydrophobic interaction resin. The resin can be eluted with a salt gradient which, when the ligand is a phenyl group, contains substantially no alcohol, and thus resulting in fractions enriched in the desired growth factors. These fractions can be separated further by means of a hydroxyapatite column. L'invention concerne un procédé d'extraction de composants bénéficiaires, en particulier de facteurs de croissance, tels que des TGF beta et IGF-1 à partir de lait. Dans ce procédé, on trouve également une phase de chromatographie interactive hydrophobe. Une résine possède un groupe butyle ou un groupe phényl comme ligand de résine interactive hydrophobe. Cette résine peut être éluée par un gradient de sel qui, lorsque le ligand est un groupe phényl, ne contient quasiment pas d'alcool et résulte donc en fractions enrichies dans les facteurs de croissance souhaités. On peut séparer ces fractions ultérieurement au moyen d'une colonne hydroxylapatite.