METHODS FOR PRODUCTION OF P-HYDROXYBENZOATE IN BACTERIA

This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to...

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Bibliographische Detailangaben
Hauptverfasser:	DUQUE, ESTRELLA, BEN-BASSAT, ARIE, GODOY, PATRICIA, RAMOS-GONZALES, M., ISABEL, RAMOS, JUAN, L
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production. This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production. L'invention concerne l'isolement d'un nouveau tonB operon du Pseudomonas putida. Ces gènes sont utilisés pour rendre les cellules plus sensibles aux antibiotiques, au toluène, au pHBA, aux composés aromatiques, aux parabènes ainsi qu'aux aminoacides aromatiques, après inactivation avec des allèles mutants spécifiques, ou plus tolérantes à ces composés, après surexpression avec un vecteur d'expression approprié. Ces observations sont importantes dans le domaine de la médecine, de la biotechnologie et de la biocatalyse. L'invention spécifie en outre un écran permettant d'identifier des gènes tolérants au pHBA, et des souches à tolérance significative au pHBA ont été identifiées. Ces souches sont importantes pour la production de pHBA.