METHOD FOR QUANTIFYING PHOSPHOKINASE ACTIVITY ON PROTEINS

The invention involves a method for measuring phosphorylation of proteins and, as such, is an indicator of protein kinase activity. The method involves the in vitro phosphorylation of a target protein but subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, inclu...

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Bibliographische Detailangaben
Hauptverfasser:	WANG, JIMIN, REAGAN, KEVIN, J, SCHAEFFER, ERIK
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention involves a method for measuring phosphorylation of proteins and, as such, is an indicator of protein kinase activity. The method involves the in vitro phosphorylation of a target protein but subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phostphorylated protein is measured by contacting it with an antibody specific for the phosphorylation sites(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to phosphorylation of Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins. L'invention porte sur un procédé de mesure de la phosphorylation de protéine servant d'indicateur de l'activité de protéine kinases. Le procédé comporte la phosphorylation in vitro d'une protéine cible, obtenu en soumettant ladite protéine (non-phosphorylée) à un mélange contenant tous les réactifs dont la phosphokinase, pour donner la forme phosphorylée de la protéine. On mesure la protéine phosphorylée en la mettant en contact avec un anticorps spécifique du ou des sites de phosphorylation. L'invention porte également sur les anticorps utiles pour mettre en oeuvre lesdits procédé, et en particulier sur la phosphorylation des protéines Tau, Rb et EGFR, et sur les anticorps spécifiques des site de phosphorylation des protéines Tau, Rb ou EGFR.