MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE
Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where t...
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| creator | MEYERING, MARK, T ARNOLD, TODD, EDWARD CHESTERSON, RICHARD, S |
| description | Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples.
L'invention concerne une membrane microporeuse composite multicouche (1, 2, 3), caractérisée en ce qu'elle comprend, dans au moins une couche (1), un matériau hydrophile, hautement électropositif, distribué uniformément, capable d'une liaison irréversible d'acide nucléique et, éventuellement, au moins une couche (2) où le matériau est associé à des acides peptide nucléiques, spécifiques d'une séquence, permettant la capture simultanée ou séquentielle, l'amplification et/ou l'identification de séquences appropriées d'acide nucléique spécifiques. L'invention concerne également des procédés d'utilisation des membranes composites selon l'invention, dans des applications basées sur la capture spécifique de séquences et/ou l'amplification et l'identification d'acides nucléiques à partir d'échantillons biologiques complexes. |
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L'invention concerne une membrane microporeuse composite multicouche (1, 2, 3), caractérisée en ce qu'elle comprend, dans au moins une couche (1), un matériau hydrophile, hautement électropositif, distribué uniformément, capable d'une liaison irréversible d'acide nucléique et, éventuellement, au moins une couche (2) où le matériau est associé à des acides peptide nucléiques, spécifiques d'une séquence, permettant la capture simultanée ou séquentielle, l'amplification et/ou l'identification de séquences appropriées d'acide nucléique spécifiques. L'invention concerne également des procédés d'utilisation des membranes composites selon l'invention, dans des applications basées sur la capture spécifique de séquences et/ou l'amplification et l'identification d'acides nucléiques à partir d'échantillons biologiques complexes.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SEPARATION ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20021212&DB=EPODOC&CC=WO&NR=02099139A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20021212&DB=EPODOC&CC=WO&NR=02099139A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MEYERING, MARK, T</creatorcontrib><creatorcontrib>ARNOLD, TODD, EDWARD</creatorcontrib><creatorcontrib>CHESTERSON, RICHARD, S</creatorcontrib><title>MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE</title><description>Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples.
L'invention concerne une membrane microporeuse composite multicouche (1, 2, 3), caractérisée en ce qu'elle comprend, dans au moins une couche (1), un matériau hydrophile, hautement électropositif, distribué uniformément, capable d'une liaison irréversible d'acide nucléique et, éventuellement, au moins une couche (2) où le matériau est associé à des acides peptide nucléiques, spécifiques d'une séquence, permettant la capture simultanée ou séquentielle, l'amplification et/ou l'identification de séquences appropriées d'acide nucléique spécifiques. L'invention concerne également des procédés d'utilisation des membranes composites selon l'invention, dans des applications basées sur la capture spécifique de séquences et/ou l'amplification et l'identification d'acides nucléiques à partir d'échantillons biologiques complexes.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SEPARATION</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2002</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyssKwjAQheFsXIj6DuMDFFq76jJOJjRgMjUXuixF4qpoob4_XhDcujrwn28tJsvKaEMKrEHPHXtOASzZo5eOQLMHx64IHeGLIUinINA5kUP6VZfwRO8XjQKUXUyePtRSbFkFYA0p0FasruO05N13N2KvKWJb5Pk-5GUeL_mWH0PP5aFsmqpuZFX_Y57asTaA</recordid><startdate>20021212</startdate><enddate>20021212</enddate><creator>MEYERING, MARK, T</creator><creator>ARNOLD, TODD, EDWARD</creator><creator>CHESTERSON, RICHARD, S</creator><scope>EVB</scope></search><sort><creationdate>20021212</creationdate><title>MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE</title><author>MEYERING, MARK, T ; ARNOLD, TODD, EDWARD ; CHESTERSON, RICHARD, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO02099139A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2002</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SEPARATION</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MEYERING, MARK, T</creatorcontrib><creatorcontrib>ARNOLD, TODD, EDWARD</creatorcontrib><creatorcontrib>CHESTERSON, RICHARD, S</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MEYERING, MARK, T</au><au>ARNOLD, TODD, EDWARD</au><au>CHESTERSON, RICHARD, S</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE</title><date>2002-12-12</date><risdate>2002</risdate><abstract>Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples.
L'invention concerne une membrane microporeuse composite multicouche (1, 2, 3), caractérisée en ce qu'elle comprend, dans au moins une couche (1), un matériau hydrophile, hautement électropositif, distribué uniformément, capable d'une liaison irréversible d'acide nucléique et, éventuellement, au moins une couche (2) où le matériau est associé à des acides peptide nucléiques, spécifiques d'une séquence, permettant la capture simultanée ou séquentielle, l'amplification et/ou l'identification de séquences appropriées d'acide nucléique spécifiques. L'invention concerne également des procédés d'utilisation des membranes composites selon l'invention, dans des applications basées sur la capture spécifique de séquences et/ou l'amplification et l'identification d'acides nucléiques à partir d'échantillons biologiques complexes.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE |
| title | MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE |
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