MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE

MODIFIED MICROPOROUS MEMBRANE FOR NON-SPECIFIC AND SEQUENCE-SPECIFIC NUCLEIC ACID CAPTURE AND METHODS OF USE

Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MEYERING, MARK, T, ARNOLD, TODD, EDWARD, CHESTERSON, RICHARD, S
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROCESSES OF PREPARING SUCH COMPOSITIONS
SEPARATION
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Disclosed herein is a multi-layered (1, 2, 3), composite microporous membrane comprising in at least one layer (1) a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer (2) where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples. L'invention concerne une membrane microporeuse composite multicouche (1, 2, 3), caractérisée en ce qu'elle comprend, dans au moins une couche (1), un matériau hydrophile, hautement électropositif, distribué uniformément, capable d'une liaison irréversible d'acide nucléique et, éventuellement, au moins une couche (2) où le matériau est associé à des acides peptide nucléiques, spécifiques d'une séquence, permettant la capture simultanée ou séquentielle, l'amplification et/ou l'identification de séquences appropriées d'acide nucléique spécifiques. L'invention concerne également des procédés d'utilisation des membranes composites selon l'invention, dans des applications basées sur la capture spécifique de séquences et/ou l'amplification et l'identification d'acides nucléiques à partir d'échantillons biologiques complexes.