METHOD AND APPARATUS FOR FRACTIONAL SEPARATION OF PROTEINS FROM PLANT MATERIAL

METHOD AND APPARATUS FOR FRACTIONAL SEPARATION OF PROTEINS FROM PLANT MATERIAL

A method of extracting and purifying recombinant proteins (44) from transgenic plant matter (11) is disclosed. Fractioning of juice (14) that has been extracted from the plant matter (11) is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (17, 19...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MOONAN, FRANCIS, BARRILLEAUX, ADAM, IRVINE, JAMES, E, MONCLIN, JEAN, P, MIRKOV, ERIK, T
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method of extracting and purifying recombinant proteins (44) from transgenic plant matter (11) is disclosed. Fractioning of juice (14) that has been extracted from the plant matter (11) is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (17, 19, 21) followed by preferably membrane type filters (25, 28, 32), ion exchange (36), membrane adsorber, and chromatographic processes. L'invention concerne un procédé permettant d'extraire et de purifier des protéines recombinantes (44) à partir d'une substance végétale transgénique (11). Le fractionnement du jus (14) extrait à partir de la substance végétale (11) est obtenu par mise en oeuvre d'un processus de filtrage à étapes multiples consistant à diminuer la porosité (17, 19, 21) suivi, de préférence, par des filtrages de type membranaire (25, 28, 32), un échange ionique (36), une adsorption membranaire et des processus chromatographiques.