ARTIFICIAL DERMIS AND PRODUCTION METHOD THEREFOR
The invention relates to an artificial dermis (1) obtained from plasma containing platelets (2) and human fibroblasts (3). Said plasma with platelets (2) is obtained by fractionating the whole blood (4) of the patient (8) by means of light centrifugation and the human fibroblasts (3) are obtained fr...
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Zusammenfassung: | The invention relates to an artificial dermis (1) obtained from plasma containing platelets (2) and human fibroblasts (3). Said plasma with platelets (2) is obtained by fractionating the whole blood (4) of the patient (8) by means of light centrifugation and the human fibroblasts (3) are obtained from a skin biopsy (5). The gelation is obtained by adding calcium. The inventive artificial dermis (1) favours a rapid growth of the keratinocytes (6) seeded on the surface thereof in order to form an artificial skin (7) that can be easily transplanted. Large areas of artificial dermis (1) are obtained from a small skin biopsy (5) and from minimum quantities of plasma with platelets (2). Said plasma is rich in cytokines and in platelet growth factors and therefore stimulates the proliferation of the seeded cells, both internally and on the surface. The artificial skin (7) thus produced can be used for the treatment of serious burns, chronic cutaneous ulcers, etc. or as a base for gene therapy through the use of genetically modified cells.
Dermis artificial (1) obtenida a partir de plasma con plaquetas (2) y fibroblastos (3) humanos. El plasma con plaquetas (2) se obtiene por fraccionamiento de la sangre total (4) del paciente (8) mediante centrifugación ligera, y los fibroblastos (3) humanos a partir de una biopsia de piel (5). La gelificación se consigue mediante la adición de calcio. Esta dermis artificial (1) permite un crecimiento rápido de los queratinocitos (6) sembrados en su superficie para constituir una piel artificial (7) que es fácilmente trasplantable. Se consiguen grandes superficies de dermis artificial (1) a partir de una pequeña biopsia de piel (5) y de cantidades mínimas de plasma con plaquetas (2), que al estar enriquecido en citoquinas y factores de crecimiento plaquetario potencia la proliferación de las células sembradas, tanto en su interior como en su superficie. La piel artificial (7) asi obtenida puede servir para el tratamiento de grandes quemados, úlceras cutáneas crónicas... o utilizarse, mediante el empleo de células genéticamente modificadas, como base para terapia génica.
L'invention concerne un derme artificiel (1) obtenu à partir de plasma contenant des plaquettes (2) et des fibroblastes (3) humains. On obtient ce plasma contenant des plaquettes (2) par fractionnement du sang total (4) du patient (8) au moyen d'une centrifugation légère et les fibroblastes (3) humains à partir d'une biopsie de peau (5). On obtient la gélification |
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