ACTIVATION OF NUCLEAR TRANSFER EMBRYOS

Methods for the activation of nuclear transferred embryos using elevated calcium levels introduced into cells, and a maturation promoting factor (MPF) inhibitor are described. Elevated calcium levels introduced into the embryo cells from a culture medium containing elevated calcium levels, in the ra...

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Bibliographische Detailangaben
Hauptverfasser:	GRUPEN, CHRISTOPHER, GERALD, NOTTLE, MARK, BRENTON
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for the activation of nuclear transferred embryos using elevated calcium levels introduced into cells, and a maturation promoting factor (MPF) inhibitor are described. Elevated calcium levels introduced into the embryo cells from a culture medium containing elevated calcium levels, in the range 2 mM to about 12 mM, followed directly and immediately by incubation with DMAP are described. Also described are animals produced from embryos so treated. L'invention concerne des méthodes d'activation d'embryons de transfert nucléaire, qui mettent en oeuvre des niveaux calciques élevés introduits dans des cellules, et un inhibiteur du facteur promoteur de maturation (MPF). L'invention concerne également l'introduction de niveaux calciques élevés dans des cellules d'embryons à partir d'un milieu de culture contenant des niveaux calciques élevés, de l'ordre de 2 mM à environ 12 mM, suivie directement et immédiatement d'une incubation avec DMAP. L'invention concerne en outre des animaux obtenus à partir d'embryons traités par les méthodes de l'invention.