REGULATED GENE EXPRESSION IN YEAST AND METHODS OF USE

The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth...

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Bibliographische Detailangaben
Hauptverfasser:	WOBBE, RICHARD, C, BAILEY, DAVID, A, MOORE, JEFFREY, B, BRADLEY, JOHN, D, THOMPSON, CRAIG, M
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides novel yeast cells comprising genes whose expression can be modulated by growth in the presence or absence of metal ions, methods for making such yeast cells, and methods of using such yeast cells for determining the requirement for expression of particular genes for the growth of viability of the yeast cells. The invention also provides methods of using such yeast cells in the isolation, screening and analysis of candidate antifungal compounds. La présente invention concerne des cellules de levure comprenant des gènes dont l'expression peut être modulée grâce à la croissance en la présence ou en l'absence d'ions métalliques, des procédés permettant de produire lesdites levures, et des procédés d'utilisation de cellules de levure de ce type pour déterminer l'expression requise de gènes particuliers permettant la croissance ou la viabilité des cellules de levure. Cette invention concerne également des procédés d'utilisation de cellules de levure de ce type pour l'isolation, le criblage et l'analyse de composés antifongiques candidats.