RECOMBINANT STAPHYLOCOCCUS AUREUS PEPTIDE DEFORMYLASE

An S.aureus peptide deformylase iron complex composition, a method of isolating the composition, and a method of using the composition to screen for compounds that interact with a peptide deformylase are provided. The composition of the invention retains greater than about 95 % of its catalytic defo...

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Bibliographische Detailangaben
Hauptverfasser:	YEM, ANTHONY, W, DEIBEL, MARTIN, R., JR, VOSTERS, ANNE, F, WOLFE, CINDY, L
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	An S.aureus peptide deformylase iron complex composition, a method of isolating the composition, and a method of using the composition to screen for compounds that interact with a peptide deformylase are provided. The composition of the invention retains greater than about 95 % of its catalytic deformylase activity after being stored at a protein concentration of greater than about 10 mg/ml in 50 % glycerol at -20 DEG C for six months. L'invention concerne une composition de complexe de fer S.aureus peptide deformylase, un procédé d'isolation de la composition, et un procédé d'utilisation de la composition en vue de cribler des composés interagissant avec un peptide deformylase. La composition de l'invention conserve plus d'environ 95 % de son activité catalytique deformylase après avoir été stockée à une concentration de protéines supérieure à environ 10 mg/ml dans 50 % de glycérol à 20 DEG C pendant six mois.