CULTURE OF CRYPTHECODINIUM COHNII FOR THE SYNTHESIS OF A POLYUNSATURATED FATTY ACID

C. cohnii is cultured in a suitable growth medium with acetic acid/acetate as the main carbon source. The acetate is provided, and replenished, by adding acetic acid to the growth medium in response to an increase in pH resulting from the utilisation of acetic acid/acetate by the C. cohnii. The C. c...

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Hauptverfasser:	SIJTSMA, LOLKE, DE SWAAF, MARTIN, E, RATLEDGE, COLIN, GRANTHAM, DAVID, JOHN, STEPHENSON, JANET, CHRISTINE, ANDERSON, ALISTAIR, JAMES, KANAGACHANDRAN, KANAGASOORIYAM
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKINGOILS ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Zusammenfassung:	C. cohnii is cultured in a suitable growth medium with acetic acid/acetate as the main carbon source. The acetate is provided, and replenished, by adding acetic acid to the growth medium in response to an increase in pH resulting from the utilisation of acetic acid/acetate by the C. cohnii. The C. cohnii produces relatively high levels of docosahexaenoic acid (DHA). A stationary phase is not essential for satisfactory DHA production. Cette invention concerne une C. cohnii cultivée dans un milieu de croissance acceptable en utilisant comme source de carbone de l'acide acétique/acétate. L'acétate est fourni puis complété en ajoutant au milieu de croissance de l'acide acétique lorsqu'on détecte une augmentation du pH suite à l'utilisation de l'acide acétique/acétate par la C. cohnii. La C. cohnii produit des quantités relativement élevées d'acide docosahexaénoïque (DHA). Une phase stationnaire n'est pas nécessaire à la production satisfaisante de DHA.